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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two further splice variants were claimed but comprehensive nucleotide sequence info is not however readily available.

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Blocking peptides are peptides that bind specifically for the focus on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.

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This guidebook outlines additional achievable optimization of cellular and immunoassay parameters to be sure the best possible effects are...

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This guidebook outlines additional doable optimization of cellular and immunoassay parameters to be certain the absolute best benefits are...

Antibodies sure to the blocking peptide not bind on the epitope within the focus on protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is specific.

Goal Details Cyclin E belongs towards the very conserved cyclin family members, whose customers are characterised by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic occasion. Cyclin E types a fancy with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary and it is degraded as cells development by S phase.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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